INHIBITORY EFFECTS OF THE EXTRACT AND FRUTICULIN A OBTAINED FROM SALVIA LACHNOSTACHYS BENTH LEAVES IN GP120-INDUCED COLD HYPERALGESIA IN MICE

JOYCE ALENCAR SANTOS, ANA CLAUDIA PICCINELLI, BRENDA ARNOLD, UBIRAJARA LANZA JUNIOR, MARIA ELIDA ALVES STEFANELLO, CÂNDIDA APARECIDA LEITE KASSUYA

Resumo


Introduction: Salvia lachnostachys Benth is endemic to southern Brazil and according to a chemical
study long-chain aliphatic acids and terpenoids are its components. The phytochemical study
demonstrated the presence of the diterpene fruticulin A and ursolic and oleanolic acids (1). It has been
validated that both Salvia lachnostachys and its compound Fruticulin A have anti-inflammatory and
antihyperalgesic activities in animal models (2). Neuropathic pain in HIV infection also involves the role of
gp120, which contributes to the hyperalgesic behavior in rodents when administered peripherally or
centrally producing the release of inflammatory cytokines (3, 4). Objectives: The present work has
investigated the antihyperalgesic effect of SLEE and Fruticulin A induced by intrathecal injection of gp120
in mice. Material and Methods: Male Swiss mice (n=6) received gp120 (300 pg) or sterile saline (naïve),
intrathecally. One hour before injections animals were treated orally with SLEE (100 mg/kg) or Fruticulin
A (3 mg/kg) or saline solution, as a control. Cold sensitivity was evaluated with acetone test after 2 and 3
hours of the injections. A syringe with 30 µL of acetone was positioned close to the back right paw of the
animal and liquid was released. Animals were observed for 20 seconds and the number of paw withdrawal
was counted (5). Results: After intrathecal administration, gp120 was not capable to decrease cold
hypersensitivity in mice when compared to naive group. SLEE but not Fruticulin A, significantly increased
cold sensitivity after 2 and 3 hours of gp120 injection, when compared with control group. Maximal
inhibition was 48±11% after 3 hours of gp120 injection. Discussion and Conclusion: Oral treatment with
SLEE but not Fruticulin A have showed antihyperalgesic effects in cold sensitivity model of nociception.
Further analyses should be performed to elucidate the mechanism of gp120 induced-hyperalgesia and
what would be SLEE mechanism of action.

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