NEUROPHARMACOLOGICAL POTENTIAL EVALUATION OF AQUEOUS EXTRACT OBTAINED FROM TWO DIFFERENT SPECIES OF MIKANIA

RENAN DONOMAE IWAMOTO, DANILO RAMOS SPESSOTO, CÂNDIDA APARECIDA LEITE KASSUYA, ALEXANDRE AUGUSTO BORGHI, ALEXANDRA CHRISTINE HELENA FRANKLAND SAWAYA

Resumo


Introduction: Mikania glomerata Spreng. and M. laevigata Sch. Bip. ex Baker, Asteraceae, popularly
known as guaco, have been widely used by traditional medicine to treat some diseases. Studies showed
some actions: bronchodilator, anti-allergic, anti-asthmatic, anti-ulcerogenic, anti-inflammatory and
smooth muscle relaxant. Objective: To evaluate neuropharmacological potential of aqueous extract
obtained from M. glomerata and M. laevigata in Marble Burying (MBT) and Forced Swimming (FST) tests
performed with C57BL6 mice. Materials and Methods: For MBT, one hour after drug administration (200
mg/kg, p.o.), each mouse was placed individually in a propylene cage (26 cm × 14 cm × 12 cm)
containing 5 cm deep sawdust and eighteen glass marbles equally spaced (3 cm from one another). After
30 minutes, mice were removed and the number of marbles covered with sawdust (at least two-thirds)
was counted. For FST, mice were forced to swim individually for 5 minutes in a glass cylinder containing
20 cm of water at room temperature after one hour of drug administration (200 mg/kg, p.o.). During these
5 minutes immobility time was considered when a mouse made no effort to escape/swim and was
registered. Results and Discussion: MBT can be used both as an indicator of anxiety-like behavior and/or
obsessive compulsive-like behavior. Our tests showed that group treated with M. laevigata extract
decreased significantly the number of buried marbles (6.3 ± 1.3) when compared to control group (12 ±
2). M. glomerata group showed no statistical difference. FST is a rodent behavioral test used to evaluate
antidepressant drugs and experimental protocols that aim to prevent or treat depressive-like states. Our
results showed a tendency to reduce immobility time for both Mikania groups, but the difference was not
statistically conclusive. Conclusion: According to our test results obtained, we conclude that M. laevigata
could be explored in other behavioral tests. Our next step is to test isolated compounds present in M.
laevigata extract like coumarin, kaurenoic acids, stigmasterol, amino groups, and others.

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Referências


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