EVALUATION OF AMIDES OBTAINED FROM PIPER AMALAGO IN MECHANICAL HYPERAGESY INDUCED BY CARRAGEENAN IN MICE
or facilitation. These events begin after the local release of inflammatory mediators and activation of
immune cells or specific receptors in the peripheral and central nervous system (Rocha et al., 2007). In
popular medicine, Piper amalago has been used as an anti-inflammatory agent and this effect has been
scientifically validated as topical anti-inflammatory agent (Sosa et al., 2002). The ethanol extract of the
aerial parts of Piper amalago (EEPA) possesses the anti-hyperalgesic actions Objective: Based on this
context, the objective of this study was to evaluate compounds isolated from EEPA such as the amides N-
[7-(3’,4’-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl] pyrrolidine (1) and N-[7-(3’,4’-
methylenedioxyphenyl)-2(E),4(E)-heptadienoyl]pyrrolidine (2) in experimental model of carrageenaninduced mechanical hyperalgesia in mice. Materials and Methods: In this model, male Swiss mice (20-25
g) were treated orally with, compound 1 (1mg/kg), compound 2 (1 mg/kg), one hour before the induction
of hypernociception by acute application of intraplantar 20µl of carrageenan (Cg) 300µg/paw. The
evaluation of the sensitivity threshold or mechanical hyperalgesia was performed using Von Frey
filaments before (baseline) and after injection (1-4 hours). The animals were placed in boxes with one side
transparent background on a platform with wire mesh, allowing access to rear paw of the animal Von Frey
filaments. Results: The treatment with 1 and 2 significantly prevented the reduction of threshold
mechanical sensitivity after 3 h, with a maximum reduction for 1 of 82 ± 6 % and for 2 of 86 ± 3 % (P <
0.001) compared with control. The same result occurred when the sensitivity was assessed 4 h after
carrageenan, with maximal inhibition for 1 of 96 ± 3 % and for 2 of 93 ± 6 % (P < 0.001) compared with
control. Conclusion and Discussion: Thus, the present study showed for the first time the compounds
responsible for EEPA anti-hiperalgesic activity when administered orally in mice. Studies are being
conducted to highlight the possible charge of the activity and its demonstrated its mechanism of action.
ROCHA, A. P. C. et al. Pain: Current Aspects on Peripheral and Central Sensitization. Rev Bras Anestesiol,
v.57, n.1, p. 94-105, 2007.
SOSA, S. et al. Screening of the topical anti-inflammatory activity of some Central American plants. J
Ethnopharmacol. 81, 211-5, 2002.
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