INHIBITORY EFFECTS OF THE EXTRACT AND FRUTICULIN A OBTAINED FROM SALVIA LACHNOSTACHYS BENTH LEAVES ON GP120-INDUCED MECHANICAL HYPERALGESIA IN MICE
ResumoIntroduction: Hyperalgesia induced by intrathecal administration of gp120 are responsible for the release
of inflammatory cytokines, such as IL-1?, TNF and IL-6, and the development of mechanical sensitivity (1,
2). Salvia lachnostachys Benth is native from Brazil which presented oleanolic and ursolic acids and
Fruticulin A in a preliminary phytochemical study. Previous report from our group has observed both
Salvia lachnostachys and its compound Fruticulin A have anti-inflammatory and antihyperalgesic activities
in animal models (3). Objectives: The present work has investigated the antihyperalgesic effect of S.
lachnostachys (SLEE) and Fruticulin A induced by intrathecal injection of gp120 in mice. Material and
Methods: Male Swiss mice (n=6) received gp120 (300 pg) or sterile saline (naïve), intrathecally. One hour
before injections animals were treated orally with SLEE (100 mg/kg) or Fruticulin A (3 mg/kg) or saline
solution, as a control. Mechanical sensitivity was determined by the paw withdrawal threshold using an
electronic analgesimeter. The device was positioned on the right hind paw of the animal and pressure was
determined, in grams, after the paw withdrawal. The measurements were performed at 2 and 3 hours
after intrathecal injection of gp120 (4). Results: After intrathecal administration, gp120 was capable to
significantly decrease mechanical hypersensitivity in mice when compared to naive group. SLEE and
Fruticulin A, significantly increased mechanical sensitivity after 2 and 3 hours of gp120 injection, when
compared with control group. Maximal inhibition were 33 and 48% for SLEE, respectively and 39 and 55%,
respectively, for Fruticulin A. Discussion and Conclusion: All results of the present work demonstrate that
when administered intrathecally, gp120 caused hyperalgesia and oral treatment with SLEE and its
compound Fruticulin A have showed mechanical antihyperalgesic effects. Thus, Fruticulin A may be
responsible for the mechanical antihyperalgesic effects of the SLEE.
Ashraf T, Jiang W, Hoque MT, Henderson J, Wu C, Bendayan R. Role of anti-inflammatory
compounds in human immunodeficiency virus-1 glycoprotein120-mediated brain inflammation. J
Moss PJ, Huang W, Dawes J, Okuse K, McMahon SB, Rice AS. Macrophage-sensory neuronal
interaction in HIV-1 gp120-induced neurotoxicitydouble dagger. Br J Anaesth. 2015 Mar;114(3):499-508.
Piccinelli AC, Figueiredo de Santana Aquino D, Morato PN, Kuraoka-Oliveira AM, Strapasson RL,
Dos Santos EP, et al. Anti-Inflammatory and Antihyperalgesic Activities of Ethanolic Extract and Fruticulin
A from Salvia lachnostachys Leaves in Mice. Evid Based Complement Alternat Med. 2014;2014:835914.
Vachon P, Millecamps M, Low L, Thompsosn SJ, Pailleux F, Beaudry F, et al. Alleviation of
chronic neuropathic pain by environmental enrichment in mice well after the establishment of chronic
pain. Behav Brain Funct. 2013;9:22.