ANTINOCICEPTIVE ACTIVITY OF THE ESSENTIAL OIL FROM Campomanesia adamantium FRUIT IN RATS

DANIELI Z. VISCARDI, CAMILA DE AZEVEDO CHAVES CORREIA, JUCICLÉIA DA SILVA ARRIGO, CLÁUDIA ANDRÉA LIMA CARDOSO, IRIANI RODRIGUES MALDONADE, CÂNDIDA APARECIDA LEITE KASSUYA, ELIANA JANET SANJINEZ-ARGANDOÑA

Resumo


Introduction: Campomanesia adamantium (Myrtaceae) is popularly known as guavira and has been
used in folk medicine as antirheumatic, antidiarrheal, hypocholesterolemic and anti-inflammatory agent
(1). At harvest, fruits are consumed in natura or processed in various preparations like jellies, liqueurs,
among others (2). Objective: The present study has evaluated the antinociceptive activity of essential
oils from peel (EOP) and seed (EOS) of C. adamantium fruits in formalin model. Material and Methods:
Essential oils were obtained from 200 g of dried peel (EOP) and seeds (EOS) from C. adamantium by
hidrodistillation using a Clevenger-type apparatus for 3 hours. Samples obtained by hydrodistillation
were analyzed by gas chromatography. Nociception was evaluated after injection of formalin (3). Sixty
minutes before, male Wistar rats (n=5) were divided in groups: dexamethasone (1mg/kg, s.c.), EOP (100
and 300 mg/kg), EOS (100 and 300mg/kg) and vehicle saline solution (0.9%), treated by oral route. One
hour after treatments, the animals received an injection of 20 µl of saline containing 2.5% of formalin in
the right hind paw. Pain reaction time (paw licking) in seconds was evaluated from 0 to 5 min (phase 1 –
neurogenic pain) and from 15 to 30 min (phase 2 - inflammatory response) after injection of formalin in
the paw which represents a tonic response to pain, accompanied by an inflammatory response. Results:
EOP and EOS produced significant antinociceptive effects in the first phase when compared to control
group. EOP and EOS, respectively at the doses of 100 and 300 mg/kg significantly reduced licking time in
the second phase of formalin test in rats. Dexamethasone (1 mg/kg) produced significant
antinociceptive activity in both phases of the formalin method. It was also found that orally
administration of EOP and EOS inhibited formalin induced paw edema. Discussion and Conclusion: The
anti-inflammatory action of C. adamantium on formalin-induced is in accordance with the results of the
antinociceptive test. Analyzes using chromatography (GC-MS) indicated the presence of limonene,
Thujopsene and ?-pinene that can be characterized as being a contributor to the anti-inflammatory
effects, suggesting their use as nutraceutical or pharmacological agent.

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Referências


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