Introduction: Caryocar brasiliense, popularly known as "pequi", is a native plant of the Brazilian cerrado (savanna) biome esteemed for its use in the local cuisine and because of its economic importance, especially among the low-income population1,2,3. Medicinal properties are also attributed to this plant, such as antinociceptive and anti-inflammatory effects3,4,5. Unfortunately, there are few studies related to this plant, and it is difficult to recognize and prove all its therapeutic potential3,5. The aim of this study was to evaluate the antinociceptive action of C. brasiliense on mechanical and thermal hyperalgesia induced by inflammation. Material and Methods: The oil obteined of fruts of C. brasiliense was tested in adult male Swiss mice, which were divided into three groups: the negative control group (0,9% saline solution), the C. brasiliense treated group (oil 300mg/kg), treated by oral route, and the positive control group (dexamethasone 1mg/kg s.c.). After 1 hour, the animals received intraplantar injection of carrageenan (300?g). Mechanical hiperalgesia (Von Frey test) and the Cold allodynia (Acetone test) were analyzed 3 and 4 hours after intraplantar injection of carrageenan6,7. The experimental procedures executed in this study were in accordance with the Ethical Principals in Animal Research and approved by the Committee for Ethics in Animal Experimentation at the UFGD (Protocol number 17/2015). Results: In the third hour of the experiment, Dexamethasone reversed 87% of mechanical hyperalgesia in relation to the negative control group (P<0.05). During the fourth hour, it was observed that C. brasiliense reversed 97% of mechanical hyperalgesia (P<0.001), while Dexamethasone reversed 84% (P<0.01). Additionally, during the third and fourth hours, Dexamethasone reversed 49% (P<0.01) and 47% (P<0.05), respectively, of Cold allodynia, compared to the negative control group. Discussion and Conclusion: C. brasiliense oil was effective in reversing mechanical hyperalgesia, proving its use as an alternative therapy for this purpose. However, its effects on termal hyperalgesia (Cold allodynia) were not statistically significant. Further studies will be necessary to determine the medicinal properties of C. brasilienses, especially those related to its anti-inflammatory effects.
JÚNIOR, José Ribamar Sousa; ALBUQUERQUE, Ulysses Paulino; PERONI, Nivaldo. Traditional knowledge and management of Caryocar coriaceum Wittm.(Pequi) in the Brazilian savanna, Northeastern Brazil1. Economic Botany, v. 67, n. 3, p. 225-233, 2013.
PINTO, L. C. L. et al. Traditional knowledge and uses of the Caryocar brasiliense Cambess.(Pequi) by “quilombolas” of Minas Gerais, Brazil: subsidies for sustainable management. Brazilian Journal of Biology, v. 76, n. 2, p. 511-519, 2016.
DE FIGUEIREDO, Patrícia Rosane Leite et al. Caryocar coriaceum Wittm.(Pequi) fixed oil presents hypolipemic and anti-inflammatory effects in vivo and in vitro. Journal of ethnopharmacology, v. 191, p. 87-94, 2016.
DA SILVA, Luiz Fernando Batista Pereira et al. Anti-inflammatory action of pequi oil associated to ultrasound in tendinitis in rats: macroscopic and histological analysis. Manual Therapy, Posturology & Rehabilitation Journal, v. 14, p. 347, 2016.
DE OLIVEIRA, Francisco Fábio Bezerra et al. Antinociceptive and anti-inflammatory effects of Caryocar coriaceum Wittm fruit pulp fixed ethyl acetate extract on zymosan-induced arthritis in rats. Journal of ethnopharmacology, v. 174, p. 452-463, 2015.
YOON, Choi et al. Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain. v.59, n. 3, p.369-76, 1994.